标签存档哺乳动物
![poly θ在DNA/RNA引物模板上的三元结构:(a) poly θ聚合酶;(B) poly θ和PolθΔL对DNA/RNA的延伸;(C) Polθ:DNA/RNA:ddGTP结构;(D) poly θ:DNA/RNA(海洋)和poly θ:DNA/DNA(橙色，4x0q)的叠加;手指和拇指子域进行重构;(E) Polθ:DNA/RNA(海洋)和Polθ:DNA/DNA(橙色，4x0q)的叠加，突出K2181的12-Å位移(蓝色框;拇指)和E2246 4.4-Å移位(灰色框;palm);(F) poly θ:DNA/RNA:ddGTP和poly θ:DNA/DNA:ddGTP结构的核酸与ddGTP的叠加;(G)顶部:poly θ中ddGTP和3 '引物末端的电子密度:DNA/RNA结构; bottom: zoomed-in image of the superposition of active sites, illustrating a different conformation of ddGTP in the Polθ:DNA/RNA (blue) and Polθ:DNA/DNA (salmon) complexes; (H) interactions between ribose 2’-hydroxyl groups of the RNA template and residues in the Polθ:DNA/RNA structure; red dashed lines, hydrogen bonds; (I) DNA/RNA used for cocrystallization with Polθ and ddGTP (top); strong electron density is present for four base pairs [nucleotides located at positions 2 to 5 (underlined) of the DNA/RNA] and two base pairs resulting from an incorporated ddGMP (2’,3’ dideoxyguanosine monophosphate) (green; position 1) and a bound unincorporated ddGTP (red; position 0) in the active site (top); interactions between Polθ and nucleic acids in Polθ:DNA/RNA:ddGTP (bottom); interactions between residues and phosphate backbone, sugar oxygen, or nucleobase are shown in blue, yellow, and green, respectively; hydrogen bonds between Polθ and ribose 2’-hydroxyl groups are indicated (boxed residues); (J) interactions between Polθ and nucleic acids in Polθ:DNA/DNA:ddGTP (4x0q); color scheme identical to (I). Image credit: Chandramouly et al., doi: 10.1126/sciadv.abf1771.](http://cdn.sci-news.com/images/2021/06/image_9759-Polymerase-Theta-195x110.jpg)
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